Supplemental Data: Proteome Analysis of Escherichia coli K-12 by Two-Dimensional Native-State Chromatography and MALDI-MS
Champion, M.M., Campbell, C.S., Siegele, D.A., Russell, D.H., Hu, J.C. (2002)



The list below is the supplemental data, available in several forms.  Clicking on the link underneath "Excel" will give you the option to 1. Save the file or 2. View the file in Excel. Clicking on the link under "HTML" will open the data up in a separate window.  

All Co-fraction Combinations
[Excel]  [HTML]

This file is sorted by proteome, and lists each protein identified and what other identified proteins
co-fractionate with it.  Proteomes 1 & 3 were performed at pH 7.50, proteomes 2 & 4 at pH 8.75.


All 4 Proteome Identifications Redundant
[Excel]  [HTML]

This is a complete list of all of the proteins identified by LC/LC MS at pH 7.50 & pH 8.75.  The list is color
coded by experiment, and sorted by SWISS-PROT ID.


Annotated 2D Gel List From pH 7.50 Gels
[Excel] [HTML]

This file contains a list of the proteins we identified by LC/LC MS at pH 7.50 indexed by anion-exchange
fraction.  The columns indicate whether or not a protein seen in the experiment also had a corresponding
spot seen on 2D gels run of the same fractions.


All Pair-Wise Combinations Seen in Multiple Proteomes
[Excel]  [HTML]

This file contains all possible pair-wise interactions of co-fractionating proteins.  Colored columns indicate
co-fractionating pairs seen at different pH's, and are designated as our 'Potential Interacting Pairs.'

Number of Times Each Protein Identified
[Excel]  [HTML]

This file contains the list of all identified proteins and how many times each individual protein was identified.


Non-Redundant Proteome List
[Excel] [HTML]

This file contains the non-redundant list of the proteins identified from the four LC/LC MS experiments with gene
and SWISS-PROT ID's.


Our Eg Values (Expression Index)
[Excel] [HTML] 

This file contains the E(g) values for our identified proteins, according to Karlin & Mrázek.


Predicted pI & MW Data
[Excel]  [HTML]

This file contains the predicted pI's and MW's for our identified proteins using the Expasy pI/MW Tool.


Spots ID From 2D Gels Not Seen In SWISS-2D DB
[Excel]  [HTML]

This file contains the list of spots correlated on 2D gels that are un-annotated in the SWISS-2D Database.


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