Publications

  1. Champion, M.M., Campbell, C.S., Siegele, D.A., Russell, D.H., Hu, J.C. (2003) Proteome Analysis of Escherichia coli K-12 by Two-Dimensional Native-State Chromatography and MALDI-MS. Mol. Micro.  2003 47(2); 383-396.[PubMed] [PDF]  [Supplemental Material]
  2. Mariño-Ramírez, L., Campbell L.,  and Hu JC (2002) Screening peptide/protein libraries fused to the lambda repressor DNA-binding domain in E. coli cells.  Methods Mol Biol.  2003. 205:235-50. [PubMed]
  3. Mariño-Ramírez, and Hu JC. (2002) Isolation and mapping of self-assembling protein domains encoded by the Saccharomyces cerevisiae genome using lambda repressor fusions. Yeast. 2002 May;19(7):641-50. [PubMed] [PDF]
  4. Hu, JC (2001) Model systems: Studying molecular recognition using bacterial n-hybrid systems. Trends Microbiol. 2001 May;9(5):219-22. Review.  [PubMed] [PDF]

  5. Zhu H, Celinski SA, Scholtz JM, Hu JC. (2001) An engineered leucine zipper a position mutant with an unusual three-state unfolding pathway. Protein Sci. 2001 Jan;10(1):24-33. [PubMed] [PDF]

  6. Mariño-Ramírez, L. and Hu, J. C. (2001) Using lambda repressor fusions to isolate and characterize self-assembling domains [CSHL Press]
  7. Hu, JC. (2000) A guided tour in protein interaction space: coiled coils from the yeast proteome.
    [PubMed] [PDF]
  8. Siegele, D.S., Campbell, L., Hu, J.C. (2000) Green fluorescent protein as a reporter of transcriptional activity in a prokaryotic system. Methods Enzymol 2000;305:499-513  [PubMed]
  9. Rieker, J., Hu, J.C. (2000) Molecular Applications of fusions to leucine zippers. Methods Enzymol 2000;328:282-96 [PubMed]
  10. Kopytek, S.J., Dyer, J.C., Knapp, G.S. & Hu, J.C.(2000) Resistance to methotrexate due to AcrAB-depndent export from Escherichia coli. Antimicrob Agents Chemother. 44:3210-3212 [PubMed] [PDF]
  11. Kopytek, S.J., Standaert, R.F., Dyer, J.C. & Hu, J.C. (2000) Chemically induced dimerization of dihydrofolate reductase by a homobifunctional dimer of methotrexate. Chemistry and Biology 7:313-21 [PubMed] 
  12. Hays LB, Chen YS, Hu JC. (2000) Two-hybrid system for characterization of protein-protein interactions in E. coli. Biotechniques 2000 Aug;29(2):288-90, 292, 294 [PubMed]
  13. Zhu H, Celinski SA, Scholtz JM, Hu JC. (2000) The contribution of buried polar groups to the conformational stability of the GCN4 coiled coil. J Mol Biol. 2000 Jul 28;300(5):1377-87 [PubMed] [PDF]
  14. Hu JC, Kornacker MG, Hochschild A. (2000) Escherichia coli one- and two-hybrid systems for the analysis and identification of protein-protein interactions Methods. 2000 Jan;20(1):80-94. Review.
    [PubMed] [PDF].
  15. Zhang, Z. Murphy, A, Hu, J.C. & Kodadek, T. (1999) Genetic selection of short peptides that support protein oligomerization. Current Biology 8: 417-420. [PubMed] [PDF]
  16. Zeng, X, Zhu, H., Lashuel, H.A. & Hu, J.C. (1997) Oligomerization properties of GCN4 leucine zipper e & g position mutants. Protein Science 6:2218-2226 [PubMed]
  17. Siegele, D.A., & Hu, J.C. (1997) Autocatalytic induction of gene expression from ParaBAD plasmids at subsaturating inducer concentrations. Proc. Natl. Acad. Sci USA 94: 8168-8172. [PubMed] [PDF]
  18. Kim, Y.-I. & Hu, J.C. (1997) Oriented DNA binding by one-armed lambda repressor heterodimers and contacts between repressor and RNA polymerase at PRM. Molecular Microbiology 25: 311-318 [PubMed]
  19. Zeng, X., Herndon, A.M. & Hu, J.C. (1997) Buried asparagines determine the dimerization specificities of leucine zipper mutants. Proc. Natl. Acad. Sci USA. 94: 3673-3678 [PubMed] [PDF]
  20. Zeng, X & Hu, J.C. (1997) Detection of tetramerization domains in vivo by cooperative DNA binding to tandem lambda operator sites. Gene 185: 745-749 [PubMed] [PDF]
  21. Kim, Y.-I. & Hu, J.C. (1995) Operator binding by lambda repressor heterodimers with one or two N-terminal arms. Proc. Natl. Acad. Sci. 92: 7510-7514.[PubMed] [PDF]
  22. Hu, J.C. (1995) Repressor fusions as a tool to study protein-protein interactions. Structure 3: 431-433 [PubMed]
  23. Hu, J.C. Newell, N.E., Tidor, B & Sauer, R.T. (1993). Probing the roles of residues at the e and g positions of the GCN4 leucine zipper by combinatorial mutagenesis. Protein Science 2: 1072-1084 [PubMed]
  24. Hu, J.C. & Sauer, R.T. (1992) The basic-region leucine-zipper family of DNA binding proteins. Nucleic Acids and Molecular Biology. vol 6, pp 82-101 F. Eckstein and D. Lilly, eds. 
  25. Hu, J.C., O'Shea, E.K., Kim, P.S. & Sauer, R.T. (1990) Sequence requirements for coiled-coils: Analysis with lambda repressor-GCN4 leucine zipper fusions. Science 250: 1400-1403. [PubMed]
  26. Reidhaar-Olson, J.O., Bowie, J.U., Breyer, R.M., Hu, J.C., Knight, K.L., Lim, W.A., Mossing, M.C., Parsell, D.A., Shoemaker, K.R. & Sauer, R.T. (1992) Random mutagenesis of protein sequences using oligonucleotide cassettes. Methods in Enzymology 208: 564-586.[PubMed]